Biochemical analysis and antimicrobial activity of the extracts of Calotropis procera and Ocimum basilicum
Naseem Khatoon, Muhammad Umar Dahot, Muhammad Rafiq, Ghulam Abass Ahmed
An experiment was conducted to determine the biochemical and antimicrobial attributes of crude plant extracts of O. basilicum and C. procera. 10% leaf and flower extracts were prepared in distilled water, acetone and methanol. Crude extracts of both C. procera and O. basilicum plants showed total sugars 6.35 - 6.5, 4.37 - 4.8 mg/ml, reducing sugars 0.26 - 0.49, and 0.22 - 0.4 mg/ml, protein 1.2 - 1.31 and, 2.3 - 2.37 mg/ml, respectively. Similarly, ten free amino acids and four sugars were identified from visualized zones through thin layer chromatography (TLC). Both the plants extracts showed bioactivity against four pathogenic bacterial and three fungal species. Leaf extracts of C. procera showed significant bioactivity against S. pneumoniae and E. coli recording 20.0 and 17.0 mm zone of inhibition. It is revealed that there may be correlation in between sugars, proteins and free amino acids with antimicrobial potential of O. basilicum and C. procera extracts.