National Journal of Advanced Research
National Journal of Advanced Research
Vol. 5, Issue 2 (2019)

Screening of the phytochemicals, antimicrobial, proximate and heavy metals compositions of Pueraria phaseoloides leave extracts

Ogugor Juliet C, Ayuk Eugene L, Osuji Amara S, Chikezie Chidimma C, Unaegbu Marcellus E

The preliminary investigation of different phytochemicals, antimicrobial, proximate and heavy metal analyses of three extracts of Pueraria phaseoloides leaves is reported. The extraction was done using three different solvents namely; distilled water, ethanol, and n-hexane respectively. Ten different phytochemicals were screened namely; alkaloids, flavonoids, phlobatannins, saponins, steroids, tannins, terpeniods, carbohydrates, phytosterols and phenolic compounds. The ethanol extract revealed the presence of only five out of the ten phytochemicals that were screened namely; alkaloids, flavoniods, saponins, tannins and carbohydrates in varying amount while steroids, phobatannins, Phytosterols, terpenoids and phenolic compounds respectively were absent. The aqueous (water) extract revealed the presence of all the phytochemicals screened while the n-hexane extract showed the presence of only three out of the ten phytochemicals namely; saponins, tannins and carbohydrates. The antimicrobial screenings of the extracts were carried out via the agar well diffusion method against one gram positive bacterium (staphylococcus aureus) and two gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa). All the extracts showed remarkable activity against the microorganisms. Proximate analysis of the leaves showed the following results; ash content (3.5%), crude fiber (16.9%), moisture content (4.40%), protein (0.34%) and carbohydrate content (74.86%). Heavy metals determination of the leaves revealed Zinc (Zn) with highest value of (16.249 ppm) followed by others Lead (Pb) (1.025 ppm), Copper, (Cu) (0.281ppm), Cadmium (Cd) (0.112 ppm), Mercury (Hg) (0.083 ppm) respectively while Arsenic (As) was absent. However, the values of all the metals were below the W.H.O acceptable limit of 0.05ppm apart from Mercury (Hg) which had a value slightly higher. The above results obtained from this study support the claim that Pueraria phaseoloides leaves have medicinal value as speculated by traditional medicine practitioners and that they are also safe for consumption.
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