National Journal of Advanced Research
National Journal of Advanced Research
Vol. 5, Issue 3 (2019)

Antibiotic susceptibility pattern of Enterococcus species from various clinical samples at a tertiary care hospital

Dr. Anu Sharma, Dr. Sonal Agarwal, Dr. NK Shaikh

Background: Enterococcus has emerged as a common organism present in hospital environment and is associated with intrinsic resistance to many antimicrobial agents like Cotrimoxazole, Aminoglycoside and Cephalosporin’s. Due to injudicious use of antibiotics, Enterococci have developed acquired resistance to drugs like tetracycline, glycopeptides and quinolones etc. The ability of Enterococci to transfer drug resistance to other bacteria like Staphylococci has made condition alarming as physicians are left with limited therapeutic options which are expensive and causes toxicity in patients taking these agents. The aim of the study is to determine the distribution of Enterococcus in various samples and to know the antibiotic susceptibility pattern of Enterococcus isolates. Material & Method: The study was conducted in Microbiology department. The study was conducted for a period of 6 months from August 2018 to Jan 2019. Various samples send to Microbiology department for culture and sensitivity was included in the study. The samples were subjected to gram stain (except blood) for provisional report and samples were inoculated on blood agar and MacConkey agar, incubated at 37⁰ C for 18-24 hours. Isolates were identified by colony characteristics and battery of biochemical tests. Antibiotic susceptibility testing was done on Mueller Hinton agar as per CLSI guidelines. Result: Out of 1250 samples received in Microbiology department for culture and sensitivity, Enterococcus species isolated were 120 (9.6%). Out of 120 Enterococci isolates E. faecalis were 112 (93.33%) while E. faecium were 8 (6.66%). Majority of Enterococci spp. isolates were obtained from urine 54.2% followed by wound swab or pus sample which accounted to 28.33%, blood 12% and vaginal swab 5%. Majority of samples isolated were from Surgery department as 62% cases were reported from there followed by Medicine department, Obstetrics and Gynecology 20% and Paediatrics 7%. Antibiotic susceptibility testing of Enterococci isolates shows 100% sensitivity to vancomycin and linezolid followed by Piperacillin tazobactam 70 (84%), Gentamycin 60 (72%), ciprofloxacin 54 (45%). Maximum resistance was reported for Ampicillin where 72% of isolates were resistance to ampicillin.
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