Optimization of the protocol for initiation, multiplication and acclimatization of sugarcane variety US-633
Naseem Khatoon, Beena Naqvi, Ghulam Abass, Faraz Ahmed
Studies were carried out for rapid micropropagation of the sugarcane variety US- 633. The explants were surface sterilized with 40% sodium hypochlorite for 20 minutes. The culture were initiated on MS medium contains BAP (0.5 to 1.5 mg/L) with kin (0.5 to 1mg/L). The multiplication of the shoots was maximum observed in 3mg/L BAP and 2mg/L kin. Rooting of the plantlets was obtained in 3mg/L NAA with 3mg/L IBA.