National Journal of Advanced Research

National Journal of Advanced Research


National Journal of Advanced Research
National Journal of Advanced Research
Vol. 4, Issue 2 (2018)

Phytochemical analysis and antibacterial activities of Aloe vera leaves extracts on bacteria isolated from diabetic foot ulcer patients from hospital environment


Unegbu Nnachetam Valentine, Ezebialu Chinenye Uzoamaka, Nkwoemeka Ndidi Ethel, Eze Ebere Mary, Ezennia Obioma Jennifer

Infected leg ulcers resulting from diabetic mellitus are a major health problem resulting in morbidity and disability. Longstanding leg ulcers are frequently colonized by microorganisms and are usually multi-drug resistant and refractory to treatment. This study was aimed at determining the phytochemical analysis and Antibacterial activities of ethanol and methanol extract of Aloe vera leaves on bacteria isolated from diabetic foot ulcer patients. 150 swabs were obtained from diabetic foot ulcer patients in the hospital. The obtained swabs were cultured on the appropriate media and incubated at 37oC for 24 hours for bacterial growth and identified through appropriate biochemical test. Solvents such as ethanol and methanol were used to extract the leaves of Aloe vera and screened for their phytochemical and antibacterial activities using standard methods. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal concentration (MBC) of the plant extracts were also determined by agar dilution methods. Standard antibiotic (Gentamicin) and DMSO (Dimethyl Sulfoxide) were used as positive and negative controls respectively. Tannins, Saponins, terpenoids, Flavonoids and reducing sugar are the phytochemical compounds present in Aloe vera leaves. The maximum antibacterial activitity was observed in ethanolic extract of Aloe vera (22mm and 24mm). The efficacy of the extracts towards inhibition of the bacteria increased with concentrations. The activity of the plant extract compared with conventional antibiotics, such as gentamicin confirmed that plant extracts are more active than constitutional antibiotics. The MIC value ranged from 0 to 100mg/ml and the MBC ranged from 0 to 200mg/ml. Extract of Aloe vera leave is cheap and was effective even against multi-drug resistant organisms as compared to the routinely used topical anti-bacterial agents. This leaf extracts could be used as a broad spectrum antibiotics in the treatment of diabetic foot infections
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