Background: Different studies have presented conflicting results concerning the effect of menopause on lipid levels. Aim: This research was conducted to find the difference in serum lipid profile between premenopausal and postmenopausal women. Patients and Methods: A cross-sectional survey of serum lipid profile in apparently two groups of healthy women in Mosul city in northern Iraq. First group involved (79) women before menopause, while the second group involved (103) women after menopause. The study was conducted during a period of 6 months commencing from 1st October 2018 to 1st April 2019. Serum lipid profile including TC, TG, and HDL-C were measured, while LDL-C and Atherogenic Index were calculated from above values. Results: Compared to premenopausal women, postmenopausal women had significantly increased levels of total cholesterol (15.7%), low density lipoprotein (LDL) cholesterol (23.3%), and triglycerides (24%). The difference did not show a trend towards an increase with the number of postmenopausal years. The high density lipoprotein (HDL) cholesterol significantly higher in premenopausal women compared with postmenopausal women (5.78%). The difference in Atherogenic index is apparent which is higher in postmenopausal women (4.59 ± 2.02 kgm/ m2) compared to premenopausal women (3.42 ± 0.66 kgm/ m2. The mean for BMI was higher in the postmenopausal compared with premenopausal women and significant differences in body weight were observed between them. The mean of age at menopause was occurring is 49, 47 years. Regarding the dyslipidimia and according to NECP (ATP III), 5 subjects (6.3%) of premenopausal women were dyslipidimic comparing with 40 subjects (39.2%) of postmenopausal women, (significant difference P<0.001). Conclusion: we conclude that the menopause is associated with potentially adverse changes in lipids and lipoproteins, independent of any effect of ageing.